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Mammalian Cell Expression System

    Bioabb has proprietary Mammalian Cell Expression System generating high-yields of mammalian proteins. This expression system is usually advised for proteins requiring mammalian post-translational modifications. Cusbaio offers a comprehensive analysis of your project to recommend expression platforms with appropriate scalability, yield, and practicality. Our good team can asses your individual requirements.
    Bioabb Mammalian Cell Expression System service offers fast production and delivery of mammalian proteins up to grams level. We offer stable cell line services if you need continued source for your protein and antibody production. Bioabb Mammalian Cell Expression System features high expression level, simple operation, short circle, low cost and straightforward cultivation with high density and large-scale are the benefits of prokaryotic expression system. The product from Mammalian Cell Expression System is the most close to the nature proteins of advanced creature in the respect of the molecular structure, character of physics and chemistry, and biological perform.

Service content Character and advantage
Expression vector construction

Cell transient transfection(Calcium phosphate coprecipitation,lipofectin transfection, electroporation):short circle,protein with high activity. We can supply experiment data and the purified product.
Stable transfection cell line(antibiotic screen):the target gene could do sustained expression with high protein activity . We can supply experiment data and the purified product.
Continue to order the same protein can enjoy the 30% ~50% discount.

Expression condition optimization
Protein expression and purification

 

Transient transfection of mammalian cell

Experiment content
Experiment flow 
Content of supply
Time
1.Prokaryotic clone
Obtain the gene
Nucleic Acid Electrophoresis picture
2 weeks
PCR product connect to the redesigned vector pcDNA3.1or psv2-dhfr 
Transformation into DH5α
Large-scale extract the recombinant plasmid
2.Transient transfection

Cells are moved into a plates and cultured
After the total cells reach in 70%~80% of the hole ,start to do transfection
48~72h later harvest the cells

 

The experiment data of Transfection

3~4 days
3.Protein expression and purify

By western blot test

Western blot 
4 weeks
Purify the expression product The purified product of 1L supernatant

Stable transfection of mammalian cell

Service content 
Experiment flow 
Content of supply 
Time
1. Prokaryotic clone
Obtain the gene
Nucleic Acid Electrophoresis picture
2 weeks
PCR product connect to the redesigned vector pcDNA3.1or psv2-dhfr
Transformation into DH5α
Large-scale extract the recombinant plasmid
2 Stable transfection

Do antibiotic screening after transfection,construct the stable transfection cell line

 

The experiment data of transfection and the cell strain with sustained and stable expression

3~5 months
3. Protein expression and purify

By western blot test

Western blot 2 weeks
Purify the expression product The purified product of 1L supernatant

    We have special vectors which  have the advantages of quick cloning target gene , facilitating purification and improving the expression level of the interest protein. 

 

Order Information

 

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i-ml.png  Manual: Polyclonal antibody preparation Service Contract