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IP and Co-IP Technical Service

    Immunoprecipitation, IP, is an assay used to purify a antigen from a sample mixture using a specific antibody attached to protein A/G agarose resin. A common method is to incubate antibody and sample, followed by addition of affinity resins to capture the antibody-antigen complex, then the eluted sample would be detected by western blot. 

    The purpose of Co-Immunoprecipitation´╝îCo-IP, is to isolate the antigen along with any proteins or ligands that are bound to it in natural condition. 

Basic process:

1. Prepare sample lysis and quantitation. 

2. Incubation of sample, antibody and protein A/G agarose. 

3. Collect precipitation, wash, eluted. 

4. Western Blot detection. 

5. Provide WB picture and the experiment report.

 

Order Information

 

i-mail.png  Email: bioabb@aol.com

i-tel.png  Tel: +86-27-87582341

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i-ml.png  Manual: Polyclonal antibody preparation Service Contract